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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 32, Number 7, September,
pp.491-498
Quantitation
of Benzodiazepines in Urine, Serum, Plasma, and Meconium by LC–MS–MS
Stephanie J. Marin1, Rebecka Coles2, Miles Merrell2, and Gwendolyn
A. McMillin2,3
1ARUP Institute for Clinical and Experimental Pathology, Salt Lake
City, Utah;
2ARUP Laboratories, Inc., Salt Lake City, Utah;
3Department
of Pathology, University of Utah School of Medicine, Salt Lake
City, Utah
A single method for confirmation and quantitation
of a panel of commonly prescribed benzodiazepines and metabolites, α-hydroxyalprazolam, α-hydroxyethylflurazepam, α-hydroxytriazolam,
alprazolam, desalkylflurazepam, diazepam, lorazepam, midazolam,
nordiazepam, oxazepam, temazepam, clonazepam, and 7-aminoclonazepam,
was developed for three specimen types, urine, serum/plasma,
and meconium. Quantitation was by liquid chromatography tandem–mass
spectrometry (LC–MS–MS) using a Waters Alliance-Quattro
Micro system. The instrument was operated in multiple reaction
monitoring mode with an electrospray ionization source in positive
ionization mode. The method was evaluated for recovery, imprecision,
linearity, analytical measurement range, specificity, and carryover.
Average recovery and imprecision (within-run, between-run, and
total % CV) were within ± 15% of the target concentrations
for urine (10 to 5000 ng/mL) and serum/plasma (10 to 2500 ng/mL)
and within ± 20% for meconium (10 to 5000 ng/g). In all,
205 patient specimens were analyzed, and the results compared
to a previous in-house gas chromatography–MS method or
LC–MS–MS results from an outside laboratory. Oxazepam
glucuronide was evaluated as a hydrolysis control for the urine
and meconium specimens.
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