| |
Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 32, Number 2, March,
pp.140-146
Accumulation,
Fractionation, and Analysis of Platinum in Toxicologically Affected
Tissues after Cisplatin, Oxaliplatin, and Carboplatin Administration
D. Esteban-Fernández[1], J.M. Verdaguer[2],
R. Ramírez-Camacho[2],
M.A. Palacios[1], and M.M. Gómez-Gómez[1],
[1]Department of Analytical Chemistry, Faculty of Chemistry, University
Complutense of Madrid, Ciudad Universitaria s/n, 28040 Madrid,
Spain and
[2]Department of Otorhinolaryngology, Hospital Universitario
Puerta de Hierro, University Autónoma of Madrid, C/ San
Martín de Porres 4, 28035 Madrid, Spain
Antitumoral Pt-containing drugs present side effects
like nephrotoxicity and ototoxicity. Several systematic experiments
have been carried out with Wistar rats treated with cisplatin,
carboplatin, and oxaliplatin to study Pt-drugs accumulation and
elimination, and Pt-biomolecule distribution in the cells and
cytosols of ear, kidney, and liver. Inductively coupled plasma-mass
spectrometry (ICP-MS) analysis shows a cisplatin accumulation
capability between oxaliplatin (the highest) and carboplatin
(the lowest). The maximum concentration of Pt in all the organs
studied was achieved around the first week after cisplatin treatment.
During the first 30 days, the elimination was very fast, decreasing
in the subsequent 60 days in all the organs. Analysis of cytosols
by liquid chromatography (LC)–ICP-MS showed an analogous
behavior. In most samples, the distribution of the three drugs
in the cellular and cytosolic fractions was similar for all the
tissues. For kidney and ear, approximately 60% and 30%, respectively,
of the metal accumulated was present in the cytosol, the cytosolic
fractions smaller than 50 KDa being especially important. Cisplatin-biomolecule
interaction strength under denaturing conditions was evaluated
by LC–ICP-MS and showed a quite strong bond.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00 each.
Visa, MasterCard and AMEX accepted.
To
order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
To order multiple copies click here.
Please indicate JAT
volume and issue along with page numbers. |
|
Home | Subscribe
| Current Issue | Back Issues
| Search | Advertise | Other Publications
| |
