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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 32, Number 5, June,
pp.344-348
Arsenic Speciation
of Arsine-Exposed Blood Samples by High-Performance Liquid Chromatography–Inductively
Coupled Plasma Mass Spectrometry and As-Adduct, A Possible Indicator
of AsH3 Exposure
Yoshiyasu Higashikawa, Yuko Kazui, Shinichi Suzuki, and Osamu
Ohtsuru
National Research Institute of Police Science, 6-3-1, Kashiwanoha,
Kashiwa-shi, Chiba-Prefecture, 277-0882, Japan
Arsine (AsH3)-exposed human blood samples
were analyzed by high-performance liquid chromatography with
inductively coupled plasma mass spectrometry (HPLC–ICP-MS)
for arsenic speciation. After exposure of human blood samples
to AsH3 vapor
for 90 min at room temperature, partial hemolysis was observed.
Plasma samples from these whole blood samples were prepared by
centrifugation at 1600 × g for 10 min and analyzed
by HPLC–ICP-MS.
In addition to arsenite [As(III); degraded from AsH3],
an unidentified arsenic species (As-adduct) was detected at a
retention time of 1.1 min. Following ultrafiltration of the plasma
samples using a molecular weight cut-off of 10 kDa, As-adduct
was not detected in the filtrate. To clarify the origin of As-adduct,
AsH3 was
added to blank plasma and As(III) was added to both whole blood
and hemolyzed blood. Although As(III) was detected in all samples,
As-adduct was not detected. These results indicate that As-adduct
was derived from erythrocytes during the process of hemolysis
by AsH3 and further suggest that As(III) and plasma
ingredients do not contribute to As-adduct production. Therefore,
the presence of As-adduct in blood could represent an indicator
of acute arsine poisoning.
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