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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 32, Number 1, January/February,
pp.73-77
Determination of VX-G Analogue in Red Blood
Cells via Gas Chromatography–Tandem Mass Spectrometry
Following an Accidental Exposure to VX
Jeffrey M. McGuire, James T. Taylor, Christopher E. Byers,
Edward M. Jakubowski, and Sandra A. Thomson
U.S. Army Edgewood CB Center, Aberdeen Proving Ground, Maryland
21010-5424
A sensitive method for determining exposure to
the chemical warfare agent VX is described in which the biomarker
ethyl methylphosphonofluoridate (VX-G) is measured in red blood
cells (RBCs) following treatment with fluoride ion using isotope-dilution
gas chromatography–tandem mass spectrometry. The analyte
was isolated via solid-phase extraction and detected using ammonia
chemical ionization in the multiple reaction monitoring mode.
A good linear relationship was obtained in the quantitative concentration
range of 4 ng/mL to 1000 ng/mL with an absolute detection limit
of < 1 pg on column. The method has been applied to the analysis
of RBCs from a laboratory worker accidentally exposed to VX vapor.
Detection and quantitation of VX-G were possible in samples taken
as late as 27 days following exposure.
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