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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 32, Number 1, January/February,
pp.63-67
A Rapid and Sensitive Technique for Assessing
Exposure to VX via GC–MS–MS Analysis
Jeffrey M. McGuire, Christopher E. Byers,
Stanley W. Hulet, Edward M. Jakubowski, and Sandra A. Thomson
U.S. Army Edgewood CB Center, Aberdeen Proving Ground, Maryland
21010-5424
A rapid and sensitive method for the determination
of the chemical warfare agent VX in plasma taken from Göttingen
minipigs® has been developed using isotope-dilution gas chromatography–tandem
mass spectrometry (GC–MS–MS). Chromatographic separation
was achieved on a 5% diphenyl/95% dimethyl polysiloxane capillary
column with a total run time of about 11 min. The analyte was
detected using ammonia chemical ionization in the multiple reaction
monitoring mode, following a simple extraction with 10% 2-propanol
in hexane. A good linear relationship was obtained in the quantitative
concentration range of 10 ng/mL to 1000 ng/mL (r2 = 0.9998) with
an average slope of 1.275 ± 0.037 (n = 7), and an absolute
detection limit of 0.4 pg on column. The average recovery for
VX was 95% in saline in the concentration range of 50–100
ng/mL. The method was successfully applied to the analysis of
VX in minipig plasma in a preliminary toxicokinetic study.
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