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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 45, Number 7, September,
pp.388-393
Microwave-Assisted Extraction and HPLC–bDAD
Determination of Drugs of Abuse in Human Plasma
Purificación Fernández[1],
Marta Lago[1], Rosa Antonia Lorenzo[2], Antonia María Carro[2],
Ana María Bermejo[1], and María Jesús Tabernero[1]
[1]Institute of Legal Medicine, Forensic Toxicology Service,
Faculty of Medicine, Santiago de Compostela, Spain and
[2]Department
of Analytical Chemistry, Faculty of Chemistry, Santiago de Compostela,
Spain
A sample preparation procedure using microwave energy
is proposed for the determination of morphine, 6-acetylmorphine,
codeine, cocaine, cocaethylene, benzoylecgonine, methadone, and
2-ethylene-1,5-dimethyl-3,3-diphenylpyrrolidine in human plasma.
A screening asymmetrical factorial design was used to identify
the most suitable extraction conditions as regards solvent, temperature,
and extraction time. The target drugs were quantified by high-performance
liquid chromatography with diode-array detection. The use of microwave
energy was found to reduce solvent consumption and extraction
time compared with solid-phase extraction. The detector response
was linear over the drug concentration range of 0.05–2.0
µg/mL in human plasma. The precision and accuracy were good,
with values less than 8% and 7%, respectively. Drug recoveries
from spiked samples ranged from 69 to 81%. The proposed method
was successfully applied to a number of forensic cases.
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