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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 45, Number 7, September,
pp.383-387
Determination of Thiocyanate in Plasma by
Ion Chromatography and Ultraviolet Detection
Elodie Saussereau, Jean-Pierre Goullé,
and Christian Lacroix
Pharmacokinetic and Toxicology Laboratory, J. Monod Hospital,
F-76083 Le Havre, France
A specific and sensitive rapid high-performance
liquid chromatographic method has been developed for determination
of thiocyanate (SCN–) in plasma. This method is based on
anion exchange chromatography after a simple ultrafiltration of
plasma diluted in water. The detection of SCN is carried out in
ultraviolet (bĒ = 210 nm). The proposed method is linear in the
range 1––30 mg/L. Intra-assay and interassay accuracy
and precision were maintained within the designated limit (<
20%). The total recovery of SCN–– varied between 97
and 103.9%. The method described should be useful for clinical
medicine. Moreover, this method was applied to the analysis of
SCN– plasma in deceased subjects, within the context of
fire, and could be of interest in forensic science as a useful
additional measurement tool for cyanide determination in blood.
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