Published: Journal of Analytical Toxicology, ISSN 0146-4760, Volume 45, Number 7, September, pp.359-368

Simultaneous Determination of Multiple Drugs of Abuse and Relevant Metabolites in Urine by LC–MS–MS
June Feng[1], Lanqing Wang[1], Ingrid Dai[2], Tia Harmon[1], and John T. Bernert[1]
[1]Emergency Response and Air Toxicants Branch, Division of Laboratory Science, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Atlanta, Georgia 30341 and
[2]Battelle Memorial Institute, 2971 Flowers Road South, Suite 233, Atlanta, Georgia 30341

A method was developed for the quantitative analysis of 30 drugs of abuse and their metabolites in urine, including opiates, barbiturates, amphetamines, cocaine, cannabinoids, phencyclidine, methadone, and benzodiazepines. This method uses solid-phase extraction (SPE) on an Oasis® HLB column followed by liquid chromatography–tandem mass spectrometry. Analytes were quantified by multiple reaction monitoring with the deuterated analogues as internal standards, using an atmospheric pressure ionization-electrospray interface. The method was validated by examining specificity, precision, accuracy, linearity, recovery, reproducibility, and detection limits. The limits of detection ranged from 9 pg/mL to 2.29 ng/mL in urine depending on the analyte. The SPE procedure was automated on a RapidTrace® workstation to increase analytical throughput, and the results obtained via automated SPE were compared to those obtained by manual SPE to examine carryover effect, precision, accuracy, recovery, and reproducibility. To evaluate method performance, 108 urine samples were collected anonymously and tested for the presence of these drugs.

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