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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 31, Number 9, November/December,
pp.596-600
TECHNICAL NOTE:Determination of Benzodiazepines
in Oral Fluid Using LC–MS–MS
Christine Moore[1], Cynthia Coulter[1], Katherine Crompton[1],
and Michael Zumwalt[2]
[1]Immunalysis Corporation, 829 Towne Center Drive, Pomona,
California 91767; and
[2]Agilent Technologies, 9780 S. Meridian
Boulevard, Englewood, Colorado 80112
A rapid, simple, highly sensitive procedure for
the simultaneous analysis of bromazepam, alprazolam, clonazepam,
lorazepam, oxazepam, diazepam, midazolam, flurazepam, flunitrazepam,
nordiazepam, triazolam, temazepam, nitrazepam, and chlordiazepoxide
in oral fluid is described, using liquid chromatography coupled
to a triple quadrupole mass spectrometer in positive electrospray
mode. Benzodiazepines in oral fluid samples (1 mL) were analyzed
using the Quantisal™ collection device, quantified using
solid-phase extraction, and detected using liquid chromatography
with tandem mass spectrometric detection. For confirmation, two
transitions were monitored and one ion ratio determined, which
was within 20% of the ratio for known calibration standards.
The limits of quantitation ranged from 0.5–5 ng/mL of neat
oral fluid; the intraday precision of the assays (n = 5) ranged
from 2.8–7.29%; and the interday precision ranged from
1.42–6.8% (n = 5). The percentage recovery of the drugs
from the collection pads ranged from a low of 81.4% for midazolam
to the highest of 90.17% for nitrazepam. The procedure can be
applied to authentic oral fluid specimens.
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