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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 31, Number 2, March 2007,
pp.98-104
Evaluation of a Rapid Oral Fluid Point-of-Care Test for
MDMA
Lisa Wilson[1], Ahmed Jehanli[1], Chris Hand[1], Gail
Cooper[1], and Robert Smith[2]
[1]Cozart Bioscience Limited, Abingdon, Oxfordshire OX14 4RY, United Kingdom
and
[2]Sheffield Hallam University, City Campus,
Howard Street, Sheffield S1 1WB, United Kingdom
Cozart Bioscience Limited has developed novel lateral flow technology
that allows the detection of drugs of abuse in biological fluids and suspect
powders. This paper describes the application of this technology for the detection
of 3,4-methylenedioxymethamphetamine (MDMA) in oral fluid. Samples (N = 370)
were obtained from the analytical laboratory at Cozart Bioscience Limited following
their routine analysis for drugs of abuse. Oral fluid samples were screened
for the presence of MDMA and methamphetamine using the Cozart® RapiScan
System (CRS) and then confirmed for the presence of amphetamines (amphetamine,
methamphetamine, MDA, MDMA, MDEA, and MBDB) by gas chromatography–mass
spectrometry (GC–MS). In addition to the detection of MDMA and methamphetamine,
the CRS cross-reacts with high levels of amphetamine to give a positive result.
One hundred and twenty-one samples screened positive using the CRS. Six of these
samples were confirmed negative for MDMA and methamphetamine, but contained
very high levels of amphetamine. Employing a screening cutoff of 45 ng/mL for
the CRS and a confirmation cutoff of 30 ng/mL for GC–MS, the sensitivity,
specificity, and accuracy were 96.6, 96.8, and 96.8%, respectively. When applying
the Substance Abuse and Mental Health Services Administration recommended confirmation
cutoff for amphetamines of 50 ng/mL, the sensitivity, specificity, and accuracy
increased marginally to 98.3, 96.9, and 97.3%, respectively.
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