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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 31, Number 2, March 2007,
pp.87-92
The Quantitation of Procaine in Equine Plasma by Liquid
Chromatography–Linear Ion Trap Mass Spectrometry
Keith D. Zientek[1], Danielle F. Anderson[2], Kirsten
Wegner[3], and Cynthia Cole[4]
[1]University of Florida, College of Veterinary Medicine, Gainesville, Florida;
[2]PPD Development, Richmond, Virginia;
[3]Anesthesia Research Lab, University of California, San Diego, San Diego,
California; and
[4]IDEXX Pharmaceuticals, Westbrook, Maine
A method for the extraction and quantitation of procaine in equine
plasma was developed for use with liquid chromatography–mass spectrometry
(LC–MS). Procaine was isolated from equine plasma by liquid–liquid
extraction at pH 11 with dichloromethane using procaine-d10 as an internal standard.
Quantitation was achieved by LC–MS using a 3-µm C-18 column coupled
to an electrospray ionization source on a linear ion-trap mass spectrometer.
The limit of detection and limit of quantitation was determined to be 50 and
200 pg/mL, respectively. The lowest limit of detection determined by previous
methods was 1 ng/mL. Administration samples were obtained as part of a larger
study to determine a regulatory limit for procaine in racehorses and procaine
concentrations were determined using this method.
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