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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 31, Number 3, April 2007,
pp.150-156
Measurement of Aflatoxin and Aflatoxin Metabolites in
Urine by Liquid Chromatography–Tandem Mass Spectrometry
Robert A. Everley[1,2], Frederic L. Ciner[1], Dongliang
Zhang[1], Peter F. Scholl[3], John D. Groopman[3], and Timothy R. Croley[1,2],
[1]Commonwealth of Virginia, Division of Consolidated Laboratories, 600 N. 5th
Street, Richmond, Virginia 23219;
[2]Virginia Commonwealth University, Department of Chemistry, 1001 W. Main Street,
P.O. Box 842006, Richmond, Virginia 23284-2006;
[3]Department of Environmental Health Sciences, Bloomberg School of Public Health,
Johns Hopkins University, Baltimore, Maryland 21205-2103
Automated immunoaffinity solid-phase extraction followed by liquid
chromatography–tandem mass spectrometry and chemical analogue internal
standardization is employed to detect and quantify the aflatoxins AFB1, AFB2,
AFG1, AFG2, and the metabolites AFM1 and AFP1 in urine. The dynamic range of
the method is nearly three orders of magnitude with limits of detection in the
low femtogram on column range. The method was validated over a 12-day period
by eight analysts. This method is suitable for agricultural, forensic, and public
health laboratories during an accidental outbreak or a chemical terrorism event
where a rapid and accurate diagnosis of aflatoxicosis is needed.
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