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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 31, Number 3, April 2007,
pp.125-131
Analysis of a Challenging Subset of World Anti-Doping
Agency-Banned Steroids and Antiestrogens by LC–MS–MS
Chad R. Borges, Nicole Miller, Melinda Shelby, Megan Hansen,
Claudia White, Matthew H. Slawson, Kim Monti, and Dennis J. Crouch
Sports Medicine Research and Testing Laboratory, Department of Pharmacology
and Toxicology, University of Utah, Salt Lake City, Utah 84108
A qualitative liquid chromatography–tandem mass spectrometry
method for the analysis of 22 sporting federation-banned anabolic agents (or
their metabolite markers) and anti-estrogens in urine that are refractory to
analysis by gas chromatography–mass spectrometry is presented. In addition,
a quantitative method built around World Anti-Doping Agency (WADA) guidelines
for the confirmatory analysis of 19-norandrosterone, the primary metabolite
of nandrolone with a WADA-specified minimum required performance limit of 1
ng/mL, is included. Hydrolysis of glucuronide conjugates, liquid–liquid
extraction, no clean-up derivatization with Girard’s Reagent P, and analysis
by quadrupole-time-of-flight mass spectrometry provide sensitivity and selectivity
well beyond that required by the WADA.
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