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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 30, Number 9, November/December 2006,
pp.679-682
Use of Capillary Electrophoresis and Poly(ethylene Oxide)
as the Coating Agent for the Determination of Substances Related to Heroin Addiction
and Treatment
Niccoló Di Pietro[1], Giorgio Placanica[2], Italbo
Fiorini[3], Clementina Manera[2], Caterina Orlando[2], Flaminia Palombo[1],
Giuseppe Saccomanni[2], Vittorio Salotti[1], and Marco Macchia[2],
[1]GeneDia S.r.l., Lammari, Lucca, Italy;
[2]Dipartimento di Scienze Farmaceutiche, Università di Pisa, Via Bonanno
6, 56126, Pisa, Italy; and
[3]ASL, n. 2, Lucca, Italy
The purpose of this study was to identify and quantify morphine,
codeine, methadone, and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine using
capillary electrophoresis in urine specimens. Adequate peak separation was achieved
using nearly neutral pH phosphate buffer and poly(ethylene oxide) as the coating
agent. This dynamic coating of the inner surface of the capillary was obtained
by rinsing with a solution containing this compound. The electroosmotic flow
and the interactions between analytes and the capillary wall surface were reduced,
while resolution and reproducibility were thus improved. Detection limits were
appropriate for usual analytical requirements in forensic laboratories.
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