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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 30, Number 9, November/December 2006,
pp.645-650
Detection of THCA in Oral Fluid by GC–MS–MS
David Day[1], David J. Kuntz[1], Michael Feldman[1], and
Lance Presley[2]
[1]LabOne, Inc., West Valley City, Utah 84120
[2]LabOne, Inc., Lenexa, Kansas 66219
A major marijuana metabolite, 11-nor-9-carboxy-tetrahydrocannabinol
(THCA), has been identified in oral fluids from donors that previously tested
positive for D9-tetrahydrocannabinol (THC). The method consisted of solid-phase
extraction of the oral fluid samples followed by gas chromatography–tandem
mass spectrometry analysis of the extracts. Testing for THCA was performed on
223 oral fluid samples previously analyzed for THC. The THCA assay was linear
from 10 to 240 pg/mL. The mean recovery of spiked THCA in oral fluid was 104%,
and precision was 4% at 20 pg/mL using fortified negative samples. This method
was rugged and robust, providing detection and quantification of THCA in oral
fluids at levels not previously reported. Results of this study showed that
THCA was detectable in 21 of 26 oral fluid samples previously reported positive
for THC. The range of concentrations from these samples was from 10 pg/mL up
to 142 pg/mL THCA.
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