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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 30, Issue 4, May 2006,
pp.262-266
The Detection of D9-Tetrahydrocannabinol (THC) and 11-nor-9-Carboxy-D9-Tetrahydrocannabinol
(THCA) in Whole Blood Using Two-Dimensional Gas Chromatography and EI-Mass Spectrometry
Rodger D. Scurlock, Greg B. Ohlson, and David K.
Worthen
AZ DPS Central Regional Crime Lab, Toxicology Unit, 2102 W. Encanto Blvd., Phoenix,
Arizona 85005
A method is described for the simultaneous analysis of D9-tetrahydrocannabinol
(THC) and its carboxylic acid metabolite, 11-nor-9-carboxy-D9-tetrahydrocannabinol
(THCA) as their trimethylsiyl derivatives using 2-dimensional chromatography
and electron ionization-mass spectrometric detection. The addition of a Deans
switch to a standard GC oven allows the use of two chromatographic columns of
differing stationary phase to greatly reduce matrix interference. The analytes
are extracted from 1 mL of whole blood by first precipitating the blood proteins
with the addition of acetonitrile followed by solid-phase extraction. The limit
of quantitation for both THC and THCA was determined to be 1.0 ng/mL. The between-run
precision at 1.0 ng/mL (N = 30) was 7.7% and 7.4% for THC and THCA, respectively.
The method is linear from 1 to 100 ng/mL.
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