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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 30, Issue 4, May 2006,
pp.258-261
High-Performance Liquid Chromatography Method for Urinary
trans,trans-Muconic Acid. Application to Environmental Exposure to Benzene
Valentina Olmos, Silvia C. Lenzken, Clara M. López,
and Edda C. Villaamil
Cátedra de Toxicología y Química Legal, Facultad de Farmacia
y Bioquímica, Universidad de Buenos Aires, Junín 956 7º (C1113ADD),
Buenos Aires, Argentina
This report describes a specific and precise high-performance
liquid chromatography (HPLC) method for the quantification of trans,trans-muconic
acid in human urine. The procedure involved a highly efficient Bond-Elut SAX
extraction with 20% acetic acid elution. The HPLC analysis used a sodium acetate/methanol
mobile phase with a C18 reverse phase column and UV detection at 265 nm. The
recovery, precision, linearity, and limits of detection and quantification of
the method were determined. Mean absolute recoveries were between 97% and 115%.
The calibration curve showed a correlation coefficient of 0.9955 and the limit
of detection was determined to be 10.8 µg/L. The method is suitable for
evaluation of occupational and environmental benzene exposure in humans. The
study of urinary trans,trans-muconic acid of two populations of children to
evaluate environmental benzene exposure is presented.
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