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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 30, Issue 4, May 2006,
pp.245-251
Quantification of Metaxalone in Human Plasma by Liquid
Chromatography Coupled to Tandem Mass Spectrometry
Ramakrishna V.S. Nirogi, Vishwottam N. Kandikere,
Manoj Shukla, Koteshwara Mudigonda, Wishu Shrivastava, and Praveen V. Datla
Biopharmaceutical Research, Suven Life Sciences, Ltd., Serene Chambers, Road
# 7, Banjara Hills, Hyderabad 500034, India
A simple, rapid, sensitive, and selective liquid chromatography–tandem
mass spectrometry (MS) method was developed and validated for the quantification
of metaxalone, a skeletal muscle relaxant, in human plasma using galantamine
as internal standard (IS). Following liquid–liquid extraction, the analytes
were separated using an isocratic mobile phase on a reverse phase C18 column
and analyzed by MS in the multiple reaction monitoring mode using the respective
[M+H]+ ions, m/z 222/161 for metaxalone and m/z 288/213 for the IS. The assay
exhibited a linear dynamic range of 50–5000 µg/L for metaxalone
in human plasma. The lower limit of quantification was 50 µg/L with a
relative standard deviation of less than 10%. Acceptable precision and accuracy
were obtained for concentrations over the standard curve range. A run time of
2.5 min for each sample made it possible to analyze more than 400 human plasma
samples per day. The validated method has been successfully used to analyze
human plasma samples for application in pharmacokinetic, bioavailability, or
bioequivalence studies.
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