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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 30, Issue 5, June 2006,
pp.317-318
SHORT COMMUNICATION: Comparison of Blood Ethanol Concentrations
in Samples Simultaneously Collected into Expired and Unexpired Venipuncture
Tubes
Donna B. Zittel and Glenn G. Hardin
Forensic Science Laboratory, Minnesota Department of Public Safety, Bureau of
Criminial Apprehension, 1430 Maryland Avenue, St. Paul, Minnesota 55106
Blood sample collection kits manufactured for the Minnesota Bureau
of Criminal Apprehension contain venipuncture tubes with an expiration date
that the tube manufacturer utilizes to ensure sufficient vacuum to fill the
tubes. Legal challenges to blood ethanol analyses that involved the use of expired
venipuncture tubes prompted a study comparing ethanol analysis results in expired
venipuncture tubes (up to 74 months beyond the expiration date) and in unexpired
venipuncture tubes. Blood samples were collected during controlled drinking
sessions using 10-mL venipuncture tubes containing 100 mg of sodium fluoride
and 20 mg of potassium oxalate and were analyzed by headspace gas chromatography.
The data obtained indicate that the ethanol concentrations in the expired and
unexpired venipuncture tubes were statistically identical.
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