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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 6, September,
pp. 377-380
TECHNICAL NOTE: Determination of Clofazimine in Leprosy
Patients by High-Performance Liquid Chromatography
R.H.C. Queiroz1,*, R.C. Pereira1, M.A. Gotardo1, D.S. Cordeiro1, and
E. Melchior, Jr.2
1Department of Clinical, Toxicological and Food Sciences Analysis, Faculty
of Pharmaceutical Sciences of São Paulo, Av. do Café s/n, 14040-903-Ribeirão
Preto, S.P., Brazil and 2Regional Sanitary Dermatology Outpatient Clinic of
Ribeirão Preto, 14080-190-Ribeirão Preto, S.P., Brazil
An original, simple, specific, and rapid high-performance liquid
chromatography assay for the determination of clofazimine in human plasma is
presented. The procedure consists of extracting the drug and the internal standard
(medazepam) from 0.5 mL plasma with dichloromethane/diisopropyl ether (1:1,
v/v) at pH 3.0, after preciptating the proteins with methanol. The drugs were
then quantitated on a reversed-phase C8 using a mobile phase consisting of
a mixture of methanol/0.25N sodium acetate buffer at pH 3.0 (74:26, v/v). The
flow-rate and wavelength were set at 1 mL/min and 286 nm, respectively. The
precision, linearity, and limit of quantitation of the method were within acceptable
limits. The method was considered adequate and could be applied in studies
involving blood level monitoring and pharmacokinetics in leprosy patients. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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