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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 6, September,
pp. 366-371
TECHNICAL NOTE: One-Step Immunoassay for Acetaminophen
and Salicylate in Serum, Plasma, and Whole Blood
Wu Song and Chao Dou
Applied Biotech, Inc., San Diego, California 92121
Acetaminophen (APAP, n-acetyl-p-aminophenol) and salicylate (ASA,
acetylsalicylic acid) are two of the most frequently overdosed drugs reported
in the emergency room. Most APAP and ASA tests are performed with analytical
instruments in laboratories, which are not always ideal for emergency situations.
A rapid membrane and gold particle-based immunoassay for the simultaneous detection
of APAP and ASA has been developed. Validation studies verified the cutoffs
for the assay as 25 µg/mL for APAP and 100 µg/mL for ASA, respectively,
and the accuracy study indicated that the agreement between the APAP and the
ASA test and reference methods is 99%. One hundred thirty-three structurally
unrelated chemicals and metabolites that may be present in human blood specimens
were tested and found not to cross-react with the test. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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