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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 6, September,
pp. 342-345
An Enzyme-Linked Immunosorbent Assay (ELISA) for Methylphenidate
(Ritalin®) in Urine
Mark G. Lewis, John G. Lewis, Peter A. Elder, and
Grant A. Moore
Canterbury Health Laboratories, Christchurch, New Zealand
A direct enyzme-linked immunosorbent assay (ELISA) for urinary
immunoreactive methylphenidate (Ritalin), in which a standard 96-well microtiter
plate is used, is described. For this ELISA, a methylphenidate-thyroglobulin
conjugate is immobilized to the microtiter plate and competes with methylphenidate
in the standard or urine sample for antibody-binding sites. After washing,
the sheep methylphenidate antibody bound to immobilized methylphenidate is
detected with peroxidase-labelled goat antisheep IgG. Following a further wash,
tetramethylbenzidine is added, color is developed, and the plate is read at
450 nm on an ELISA plate reader. This method is unaffected by drugs of abuse
and is suitable for routine use in the toxicology laboratory. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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