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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 7, October 2003,
pp. 513-516
TECHNICAL NOTE: Analysis of Fentanyl and Norfentanyl in
Human Plasma by Liquid Chromatography–Tandem Mass Spectrometry Using
Electrospray Ionization
J. Day, M. Slawson, R.A. Lugo, and D. Wilkins
Center for Human Toxicology, University of Utah, Salt Lake City, Utah 84112
This report describes a sensitive and specific high-performance
liquid chromatography (HPLC)–electrospray ionization-tandem mass spectrometry
(MS–MS) method for the detection of subnanogram concentrations of fentanyl
and its metabolite norfentanyl in human plasma. The assay was based on a liquid–liquid
extraction of 0.5 mL of human plasma, with a lower limit of quantitation (LLOQ)
of 0.05 ng/mL. Sample extracts were analyzed using a ThermoQuest TSQ® MS–MS
interfaced with a Hewlett-Packard series 1100 HPLC and a Phenomenex® (30
x 2.00-mm, 5 µ Luna C18(2)) column. The intra-assay precision and accuracy
ranged from 2.1 to 12.5% for both analytes at concentrations of 0.1, 0.5, 1.0,
and
10 ng/mL. The interassay accuracy and precision ranged from 7.34 to 10.95%.
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