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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 8, November/December,
pp. 569-573
Quantitative Identification of Atrazine and its Chlorinated Metabolites in
Plasma
Jill M. Brzezicki, Melvin E. Andersen, Brian K. Cranmer, and John D. Tessari
Department of Environmental and Radiological Health Sciences, Colorado State
University, Ft. Collins, Colorado 80523
The objective of this study was to develop an analytical method to detect
and quantitate the chlorotriazine herbicide atrazine (ATRA), and its chlorinated
metabolites [desethylatrazine (DE-ATRA), desisopropylatrazine (DI-ATRA), and
diaminochlorotriazine (DACT)] in plasma. Control plasma separated from whole
rat blood was fortified with known concentrations of ATRA, DE-ATRA, DI-ATRA,
and DACT. These compounds were extracted from the plasma using a liquid–liquid
extraction technique, and the resulting extracts were derivatized with tetrabutyl
ammonium hydroxide and methyl iodide to produce methylated derivatives of ATRA
and its chlorinated metabolites. Derivatized samples and standards were analyzed
using gas chromatography–mass spectrometry with selected ion monitoring.
Recoveries of fortified plasma samples ranged from 84% to 97% and were validated
to 100 ng/mL. This analytical method was subsequently verified in a small-scale
animal study to determine time course concentrations of chlorotriazines in
plasma following a single oral gavage dose of ATRA to female Sprague Dawley
rats. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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