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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 2, March 2003,
pp. 95-102
Determination of Methamphetamine and Its Metabolites Incorporated
in Hair by Column-Switching Liquid Chromatography–Mass Spectrometry
Akihiro Miki*, Munehiro Katagi, and Hitoshi Tsuchihashi
Forensic Science Laboratory, Osaka Prefectural Police Headquarters, 1-3-18 Hommachi,
Chuo-ward, Osaka 541-0053, Japan
An automated column-switching
liquid chromatographic– electrospray
ionization-mass spectrometric (LC–ESI-MS) method has been established for
the determination of methamphetamine (MA) and its metabolites, amphetamine (AP),
and p-hydroxymethamphetamine (p-OH-MA) in hair. The combination of an N-vinylacetamide-containing
hydrophilic polymer online extraction column, an SCX semi-micro LC column, and
an electrospray ionization interface provided the successful concentration, separations,
and highly sensitive MS determinations of these analytes in a hair extract without
tedious sample pretreatments. The limits of detection of these analytes were
0.02 ng/mg and 0.1–0.2 ng/mg in the selected-ion monitoring (SIM) and full-scan
modes, respectively, when using a 100-µL hair extract sample that corresponds
to a 2.5-mg sample of hair. The calibration curves using dibenzylamine as an
internal standard were linear up to 30 ng/mg hair equivalents for all these analytes
in the SIM mode. p-OH-MA, the detection of which in MA users’ hair had
not been previously reported, was detectable in all 22 hair specimens/sections
from which 1 ng or more of MA was detected per milligram hair. The amount of
p-OH-MA detected per milligram of hair is presented with those of MA and AP among
the MA users population. The detection of AP and p-OH-MA, in addition to the
parent drug MA with reasonable ratios, was found to be a useful indicator for
distinguishing internal MA incorporation from external contamination. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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