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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 2, March 2003,
pp. 78-87
Development of a Rapid and Sensitive Method for the Quantitation
of Amphetamines in Human Plasma and Oral Fluid by LC–MS–MS
M. Wood[1], G. De Boeck[2], N. Samyn[2], M. Morris[1],
D.P. Cooper[1], R.A.A.
Maes[3], and E.A. De Bruijn[3]
[1]Micromass U.K. Limited, Atlas Park, Simonsway, Wythenshawe, Manchester M22
5PP,
United Kingdom;
[2]National Institute of Criminalistics and Criminology (NICC),
Section Toxicology, Vilvoordsesteenweg 98, 1120 Brussels, Belgium; and
[3]Utrecht
Institute of Pharmaceutical Sciences (UIPS), Department of Human Toxicology,
University of Utrecht, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
Target analysis of amphetamines
in biological samples is of great importance for clinical and forensic toxicologists
alike. At present,
most laboratories analyze such samples by gas chromatography–mass spectrometry.
However, this procedure is labor-intensive and time-consuming, particularly as
a preliminary extraction and derivatization are usually unavoidable. Here we
describe the development of an alternative method. Amphetamines were isolated
from human plasma and oral fluid using a simple methanol precipitation step and
subsequently analyzed using reversed-phase liquid chromatography– tandem
mass spectrometry. Quantitation of the drugs was performed using multiple reaction
monitoring. The developed method, which requires only 50 µL of biological
sample, has a total analysis time of less than 20 min (including sample preparation)
and enables the simultaneous quantitation of 3,4-methylenedioxymethamphetamine,
3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, amphetamine,
methamphetamine, and ephedrine in a single chromatographic run. Limits of detection
of 2 µg/L or better were obtained. The method has been validated and subsequently
applied to the analysis of plasma and oral fluid samples collected from current
drug users. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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