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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 2, March 2003,
pp. 106-109
TECHNICAL NOTE: Quantitation of 11-nor-D9-Tetrahydrocannabinol-
9-carboxylic acid with GC–MS in Urine Collected for Doping Analysis
K.J.S. De Cock[1], F.T. Delbeke[1], D. De Boer[2],
P.
Van Eenoo[1], and K. Roels[1]
[1]Doping Control Unit, Department of Pharmacology, Pharmacy and Toxicology,
Faculty
of Veterinary Science, Ghent University, Salisburylaan 133, B-9820 Merelbeke,
Belgium and
[2]Laboratório de Análises de Dopagem e Bioquímica,
Lisbon, Portugal
An accurate, reproducible, and
validated gas chromatography–mass
spectrometry (GC–MS) method for the quantitation of 11-nor-D9-tetrahydrocannabinol-9-carboxylic
acid (THC-COOH), the major metabolite of D9-tetrahydrocannabinol, in urine is
described. Extraction was performed with n-hexane/ethyl acetate. Deuterated THC-COOH
was used as the internal standard. The GC–MS analysis was done by selected
ion monitoring. No interferences were detected in 20 blank urine samples of different
origin. The calibration curve was found to be linear over the range of 10–100
ng/mL. The calculated limits of detection and quantitation were 1.0 ng/mL and
1.7 ng/mL, respectively. Results of positive findings for cannabis use in doping
control in Flanders and Portugal in the period of 1997–2000 are commented.
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