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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 1, January/February
2003,
pp. 43-46
TECHNICAL NOTE: An Improved HPLC Analysis of the Metabolite
Furoic Acid in the Urine of Workers Occupationally Exposed to Furfural
Z.B. Tan, C.E. Tonks, G.E. O’Donnell, and R. Geyer
Laboratory Services Unit, WorkCover Authority of New South Wales, 5A Pioneer
Avenue, Thornleigh, NSW, 2120, Australia
An improved high-performance liquid chromatographic (HPLC) method
for the analysis of the metabolite furoic acid in the urine of workers occupationally
exposed to furfural is described. The procedure involved an alkaline hydrolysis
step followed by solvent extraction using ethyl acetate. HPLC analysis used
an acidic acetonitrile/water mobile phase with a C18 column and ultraviolet
detection. The overall relative recovery of furoic acid in urine was found
to be 98.8% with a relative standard deviation of 9.7%. The limit of quantitation
was determined to be 0.01 mmol/L.
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