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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 27, Number 1, January/February
2003,
pp. 1-6
Quantitative Determination of the Hydrolysis Products
of Nitrogen Mustards in Human Urine by Liquid Chromatography–Electrospray
Ionization Tandem Mass Spectrometry*
Sharon W. Lemire, David L. Ashley, and Antonia
M. Calafat
Division of Laboratory Sciences, National Center for Environmental Health/Centers
for Disease Control and Prevention, Atlanta, Georgia
Nitrogen mustards are a public health concern because of their
extreme vesicant properties and the possible exposure of workers during the
destruction of chemical stockpiles. A sensitive, rapid, accurate, and precise
analysis for the quantitation of ultratrace levels of N-ethyldiethanolamine
(EDEA) and N-methyldiethanolamine (MDEA) in human urine as a means of assessing
recent exposure to the nitrogen mustards bis(2-chloroethyl)ethylamine and bis(2-chloroethyl)methylamine,
respectively, was developed. The method was based on solid-phase extraction,
followed by analysis of the urine extract using isotope-dilution high-performance
liquid chromatography–mass spectrometry with TurboIonSpray® ionization
and multiple-reaction monitoring. The method limits of detection were 0.41
ng/mL for EDEA and 0.96 ng/mL for MDEA in 1 mL of urine with coefficients of
variation < 10% for both compounds.
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