Published: Journal of Analytical Toxicology, ISSN 0146-4760, Volume 27, Number 5, July/August, pp. 304-308

Simultaneous Plasma Lamotrigine Analysis with Carbamazepine, Carbamazepine 10,11 Epoxide, Primidone, Phenytoin, Phenobarbital, and PEMA by Micellar Electrokinetic Capillary Chromatography (MECC)
Fernando M. Lanças* and Marlene A. Sozza
University of São Paulo, Institute of Chemistry of São Carlos, São Carlos, Brazil
Maria E.C. Queiroz
University of Ribeirão Preto, Course of Biological Science, Department of Toxicology, Ribeirão Preto, Brazil

The determination of lamotrigine (LTG) simultaneously with carbamazepine (CBZ), carbamazepine 10,11 epoxide (CBZ-E), primidone (PRM), phenytoin (PHT), phenobarbital (PB), and 2-phenyl-2-ethyl-malonamide (PEMA) in human plasma was developed using micellar electrokinetic capillary chromatography (MECC) with a diode-array detector. The reproducibility of both separation and quantitation with MECC analysis were appropriate for the intra- and interassay coefficients. The evaluated drugs concentration intervals of LTG, 0.5–10.0 µg/mL; CBZ, 1.0–16.0 µg/mL; PEMA, 1.0–20.0 µg/mL; PB, 1.0–60.0 µg/mL; PRM, 1.0–20.0 µg/mL; PHT, 0.7–40.0 µg/mL; and CBZ-E, 1.0–14.0 µg/mL were linear with correlation coefficients higher than 0.987 and coefficients of the variation of the points of the calibration curve lower than 10%. The limit of quantitation of the investigated drugs in plasma varied from 0.5 to 1.0 µg/mL, depending upon the drug. The MECC technique was sensitive enough to work with microsamples into the subtherapeutic, therapeutic, and toxic concentrations, as well as showed to be simple and efficient when applied to monitoring therapeutic drugs in patients treated with a combination of lamotrigine and other antiepileptic drugs such as hepatic enzyme-inducing agents.

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