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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 6, September 2002,
pp. 360-364
TECHNICAL NOTE: Determination of Cobalt in Urine by FI-ICP-AES
with Online Preconcentration
Gustavo M. Farias[1], Rodolfo G. Wuilloud[1], Susana Moyano[1],
José A. Gásquez1, Roberto A. Olsina[1],[2], and Luis D. Martinez[1],[2]
[1]Department of Analytical Chemistry, Faculty of Chemistry, Biochemistry and
Pharmacy, National University of San Luis, P.O. Box 375, 5700 San Luis, Argentina
and
[2]Consejo Nacional de Investigaciones Científicas y Técnicas
(CONICET), Rivadavia 1917, CP C1033 AAJ, Cuidad de Buenos Aires, Argentina
A method for the preconcentration
and determination of cobalt in human urine samples was developed. The online
preconcentration and determination were attained using inductively coupled plasma
atomic emission spectromety (ICP-AES) coupled to a flow injection (FI) method.
Cobalt was retained on an Amberlite XAD-7 resin as cobalt-2-(5-bromo-2-pyridylazo)-5-diethylaminophenol
complex at pH 9.5. Cobalt was removed from the microcolumn with perchloric acid.
A sensitivity enhancement factor of 90 was obtained with respect to cobalt determination
by ICP-AES without preconcentration. The value of detection limit for the preconcentration
method proposed was 25 ng/L. The precision for 10 replicate determinations at
the 5 mg/L (mean ± SD, 5.1 ± 0.14) Co level was 2.7% relative
standard deviation, calculated from the peak heights obtained. The calibration
graph preconcentration method for cobalt was linear with a correlation coefficient
of 0.9994 from approximately 0.25 mg/L up to at least 100 mg/L. The method was
successfully applied to the determination of cobalt in human urine samples. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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