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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 6, September 2002,
pp. 347-354
Extraction of Amphetamine and Methamphetamine from Urine
Specimens with Cerex Polycrom™ Clin II Solid-Phase Extraction Columns and
the Speedisk™ 48 Pressure Processor
Melissa Churley, Paul V. Robandt, Judith A. Kuhnle, Timothy
P. Lyons, and Mark R. Bruins
Tripler Forensic Toxicology Drug Testing Laboratory, Building 40, 1 Jarrett
White Road, Tripler Army Medical Center, Hawaii 96859
Extraction of amphetamine
and methamphetamine in urine was investigated using Cerex Polycrom Clin II solid-phase
extraction columns and the Speedisk 48 Pressure Processor as a replacement for
our liquid–liquid procedure. Linearity for urine standards extracted with
the Cerex-Speedisk method ranged from 50 ng/mL for methamphetamine and from
150 ng/mL for amphetamine to 10,000 ng/mL for both. The mean recovery at the
500-ng/mL cutoff for three different lots of columns was 96.4% for AMP and 95.7%
for MET. The mean of the within-run means for three batches was 495.4 ng/mL
with a coefficient of variation (CV) of 1.2% or less for amphetamine and 496.4
ng/mL for methamphetamine with a CV of 1.7% or less. Thirty-six specimens containing
amphetamine and the same number for methamphetamine were analyzed by both the
Cerex-Speedisk and liquid–liquid methods. The correlation for specimens
containing amphetamine gave an r2 of 0.9986 with a slope of 0.99; for methamphetamine,
the r2 was 0.9997 with a slope of 0.98. The Cerex-Speedisk method cut extraction
time in half, was less costly, and greatly reduced the volume of hazardous waste. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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