| |
Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 7, October 2002,
pp. 500-503
An Evaluation of the OnTrak Testcup®-er On-Site Urine
Drug-Testing Device for Drugs Commonly Encountered from Emergency Departments
Michelle R. Peace[1], Justin L. Poklis[1], Lisa
D. Tarnai[2], and Alphonse Poklis[1]
[1]Department of Pathology, Medical College of Virginia Campus at Virginia Commonwealth
University, Richmond, Virginia 23298-0165
[2]Scientific
Testing Laboratories, Inc., Richmond, Virginia 23236
We evaluated the performance
of the Roche OnTrak Testcup-er (TC-er), an on-site drug-testing device, for
the detection of amphetamines (AMP), barbiturates (BRB), benzodiazepines (BNZ),
benzoylecgonine (BE), and opiates (OPI) in urine specimens from hospital emergency
departments. This device utilizes a competitive binding microparticle immunoassay
to simultaneously determine the presence of the following drugs or drug classes
in urine at and above the following cut-off concentrations: AMP, 1000 ng/mL;
BRB, 200 ng/mL; BNZ 200 ng/mL; BE, 300 ng/mL; and OPI, 300 ng/mL. One hundred
forty-nine urine specimens received from emergency departments were simultaneously
tested by the EMIT® II monoclonal immunoassay (Emit) and TC-er. Specimens
yielding discordant results were analyzed by gas chromatography–mass spectrometry.
There was a 98% (146/149) agreement of results between the methods for the presence
or absence of drugs. TC-er yielded 110 positive drug findings in 79 specimens,
and Emit yielded 102 positive drug findings in 76 specimens. There was a 97%
agreement between the methods for positive findings of at least one drug. The
majority of discordant results were due to variations in selectivity of the
assays for phenobarbital and BNZ metabolites. TC-er yielded a single false-positive
result for amphetamines, and barbiturates, possibly three false-positive benzodiazepine
results, and a single false-negative opiate finding. This represented four certain
errors out of 745 total results, or a 0.4% error rate. If the three false-positive
BNZ results were also considered, the error rate would be 0.9%. TC-er demonstrated
acceptable linearity around the cut-off concentration of each drug. The TC-er
was determined to be a reliable device for rapid on-site testing of emergency
department urine specimens. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00 each.
Visa, MasterCard and AMEX accepted.
To
order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
To order multiple copies click here.
Please indicate JAT
volume and issue along with page numbers. |
|
Home | Subscribe
| Current Issue | Back Issues
| Search | Advertise | Other Publications
| |
