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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 7, October 2002,
pp. 430-437
Quantitative Analysis of Selegiline and Three Metabolites
(N-Desmethylselegiline, Methamphetamine, and Amphetamine) in Human Plasma by
High-Performance Liquid Chromatography–Atmospheric Pressure Chemical Ionization-Tandem
Mass Spectrometry
Matthew H. Slawson, James L. Taccogno, Rodger L.
Foltz, and David E. Moody
Center for Human Toxicology, Department of Pharmacology and Toxicology, University
of Utah Health Sciences Center, Salt Lake City, Utah 84112
This report describes a sensitive
and specific high-performance liquid chromatography-atmospheric pressure chemical
ionization–tandem mass spectrometry method for the detection of subnanogram
concentrations of selegiline and its three principle metabolites, N-desmethylselegiline,
methamphetamine, and amphetamine, in human plasma. The assay has a dynamic range
of 0.1–20 ng/mL for selegiline and N-desmethylselegiline (norselegiline)
and 0.2–20 ng/mL for methamphetamine and amphetamine. The inter- and intra-assay
precision and accuracy varied by less than 11% for all analytes at 0.3, 2.5,
and 15 ng/mL and less than 16% at the lower limit of quantitation (0.1 ng/mL
for selegiline and norselegiline; and 0.2 ng/mL for methamphetamine and amphetamine).
Selegiline and its metabolites showed no significant loss in quantitative accuracy
after three freeze/thaw cycles or after up to 6 h at room temperature prior
to extraction. Extracted plasma samples retained quantitative accuracy after
storage for at least 7 days at –20°C or up to 70 h at room temperature.
Methanolic stock solutions were stable for at least 6 h when kept at room temperature
or at least 90 days when kept at –20°C. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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