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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 8, November/December,
pp. 575-581
TECHNICAL NOTE: Determination of D9-THC
in Whole Blood using Gas Chromatography–Mass Spectrometry
Mark Hok Chi Chu and Olaf H. Drummer
Victorian Institute of Forensic Medicine and Department of Forensic Medicine,
Monash University, 57-83 Kavanagh Street, Southbank 3006, Victoria, Australia
A simple and reliable liquid–liquid extraction method for
the determination of D9-tetrahydrocannabinol (THC)
in whole blood utilizing gas chromatography–mass spectrometry in electron
impact mode is described. The substance is derivatized with pentafluoropropionic
anhydride in pentafluropropanol. The limit of detection is 0.5 ng/mL for a 1-mL
specimen, with recovery greater than 70%. The intra-assay coefficient of variation
(CV) is 3.1% to 5.2%, and the interassay CV is 6.4% to 9.5%, calculated at THC
concentrations of 1, 5, and 25 ng/mL. The accuracy is between 95 and 97%. The
optimization of extraction and derivatization conditions is detailed.
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