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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 4, May/June
pp. 222-227
Simultaneous Determination of Cardenolides by Sonic Spray
Ionization Liquid Chromatography– Ion Trap Mass Spectrometry—A Fatal
case of Oleander Poisoning*
Tomonori Arao[1], Chiaki Fuke[1], Hajime Takaesu[1],
Masakazu Nakamoto[2], Yasumasa Morinaga[1], and Tetsuji Miyazaki[1]
[1]Department of Legal Medicine, School of Medicine, University of the Ryukyus,
207 Uehara, Nishihara, Okinawa 903-0215, Japan
[2]Department of Emergency Medicine, Okinawa Chubu Hospital, 208-3 Miyazato,
Gushikawa, Okinawa 904-2293, Japan
Simultaneous determination
of oleandrin and its three related compounds, desacetyloleandrin, oleandrigenin,
and gitoxigenin in blood by using liquid chromatography–three-dimensional
quadrupole mass spectrometry (LC–3DQMS) system equipped with sonic spray
ionization (SSI) interface was conducted. This analyzing method was suitable
for all of these compounds except gitoxigenin. The limits of detection of oleandrigenin
and desacetyloleandrin from blood were 2 ng/mL and that of oleandrin was 3 ng/mL.
The calibration curves for oleandrin, desacetyloleandrin, and oleandrigenin
were linear in the range of 5–100 ng/mL. The coefficients of variation
of oleandrin, desacetyloleandrin, and oleandrigenin in the blood were satisfactory
ranging from 1.6% to 4.1%. This analysis method was applied to a fatal case
of oleander poisoning. As a result of liquid chromatography–mass spectrometry
(LC–MS) analysis, oleandrin was detected in heart blood and cerebrospinal
fluid. Desacetyloleandrin, oleandrigenin, and gitoxigenin were not detected.
In order to make identification of oleandrin reliable, LC–MS–MS analysis
was performed. The concentrations of oleandrin found in the heart blood and
cerebrospinal fluid were 9.8 and 10.1 ng/mL, respectively. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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