| |
Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 4, May/June
pp. 216-221
A Simple Method for the Determination of Ethylene-thiourea
(ETU) in Biological Samples
Ikram Debbarh and Nicholas Moore†
Department of Pharmacology, EA 525, Université Victor Segalen, 33076
Bordeaux, France
A direct, simple, and rapid
high-performance liquid chromatographic method has been developed for the determination
of ethylene-thiourea (ETU) in biological fluids. Samples were chromatographed
on a Lichrosorb RP8 (5 µm) column after extraction with dichloromethane.
The mobile phase was a mixture of hexane/isopropyl alcohol/ethyl alcohol (93:6:1
v/v) added with 0.6 mL/L butylamine. Detection was done with a UV detector set
at 243 nm. This method was validated to standard criteria. Calibration curves
for ETU in 100 µL of 0.9% saline, 500 µL plasma, and 10 mL urine
were linear (r2 > 0.99) from 0.05 to 30 µg/mL, 0.025 to 30 µg/mL,
and 1 to 100 ng/mL, respectively. The lower limit of detection was 20 ng/mL
in plasma, 25 ng/mL in 0.9% saline, and 0.5 ng/mL in urine. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please indicate JAT
volume and issue along with page numbers. |
|
Home | Subscribe
| Current Issue | Back Issues
| Search | Advertise | Other Publications
| |
