| |
Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 4, May/June
pp. 205-210
An Online Automatic Sample Cleanup System for the Quantitative
Detection of the Benzene Exposure Biomarker S-Phenylmercapturic Acid in Human
Urine by Electrospray Ionization Tandem Mass Spectrometry
Pao-Chi Liao*, Chien-Ming Li, Lung-Cheng Lin, and
Chien-Wen Hung
Department of Environmental and Occupational Health, National Cheng Kung University
College of Medicine, 138 Sheng-Li Road, Tainan 704, Taiwan
Tung-Sheng Shih
Division of Method Development and Analysis, Institute of Occupational Safety
and Health, No. 99, Lane 407, Hengke Road, Shijr City 221, Taipei, Taiwan
An online automatic sample
cleanup system was developed for use with electrospray ionization tandem mass
spectrometry (ESI-MS–MS) for the quantitative detection of the benzene
exposure biomarker S-phenylmercapturic acid (S-PMA) in human urine. The sample
clean-up system was constructed with an autosampling device, a reversed-phase
C18 trap cartridge, a two-position switching valve, and controlling computer
software and hardware. The sample cleanup system was interfaced directly with
the ESI source of a triple-stage-quadrupole MS using multiple reaction monitoring
of negative product ions derived from S-PMA and the internal standard as the
detection mode. The calibration curve was linear using human urine spiked at
concentrations from 0.25 to 100 mg/L S-PMA (R2 = 0.997). The detection limit
of the analytical system for neat S-PMA standard solution was 0.04 µg/L,
whereas the detection limit was estimated to be lower than 0.35 µg/L for
a urine matrix containing trace amounts of S-PMA. Without tedious manual sample
cleanup procedures, the analytical system is fully automatic and therefore useful
for high-throughput urinary S-PMA determination. With the selectivity and the
sensitivity provided by MS–MS detection, the analytical system can be used
for high-throughput and accurate determination of S-PMA levels in human urinary
samples as a biomarker for benzene exposure. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please indicate JAT
volume and issue along with page numbers. |
|
Home | Subscribe
| Current Issue | Back Issues
| Search | Advertise | Other Publications
| |
