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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 4, May/June
pp. 193-200
LC–MS Analysis of Human Urine Specimens for 2-Oxo-3-Hydroxy
LSD: Method Validation for Potential Interferants and Stability Study of 2-Oxo-3-Hydroxy
LSD Under Various Storage Conditions
Kevin L. Klette, Carl K. Horn, and Peter R. Stout
Navy Drug Screening Laboratory, P.O. Box 113, Bldg. H-2033, Naval Air Station,
Jacksonville, Florida 32212
Cynthia J. Anderson
Navy Drug Screening Laboratory, 34425 Farenholt Avenue Suite 40, San Diego,
California 92134-7040
2-Oxo-3-hydroxy lysergic acid
diethylamide (O-H-LSD), a major LSD metabolite, has previously been demonstrated
to be a superior marker for identifying LSD use compared with the parent drug,
LSD. Specifically, O-H-LSD analyzed using liquid chromatography– mass spectrometry
has been reported to be present in urine at concentrations 16 to 43 times greater
than LSD. To further support forensic application of this procedure, the specificity
of the assay was assessed using compounds that have structural and chemical
properties similar to O-H-LSD, common over-the-counter products, prescription
drugs and some of their metabolites, and other drugs of abuse. Of the wide range
of compounds studied, none were found to interfere with the detection of O-H-LSD
or the internal standard 2-oxo-3-hydroxy lysergic acid methyl propylamide. The
stability of O-H-LSD was investigated from 0 to 9 days at various temperatures,
pH conditions, and exposures to fluorescent light. Additionally, the effect
of long-term frozen storage and pH was investigated from 0 to 60 days. There
was no significant loss of O-H-LSD under both refrigerated and frozen conditions
within the normal human physiological pH range of urine (4.6–8.4). However,
significant loss of O-H-LSD was observed in samples prepared at pH 4.6–8.4
and stored at room temperature or higher (24–50°C). Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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