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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26,
Number 1, April 2002, pp. 67-72
Determination of Methemoglobin and Total Hemoglobin in
Toxicological Studies by Derivative Spectrophotometry
A. Cruz-Landeira, M.J. Bal, O. Quintela, and M.
López-Rivadulla
Forensic Toxicology Service, Legal Medicine Institute, University of Santiago
of Compostela, San Francisco s/n, 15705.Santiago of Compostela, Spain
A method for the determination of methemoglobin in the presence
of other hemoglobin subforms (i.e., oxy-, deoxy-, and carboxyhemoglobin) by
use of derivative spectrophotometry is proposed. The method, which uses the
first-derivative of the spectrum at 645 nm, is straightforward and expeditious,
so it is of special interest to forensic toxicology laboratories as it allows
the simultaneous determination of the methemoglobin saturation percentage and
the hemoglobin concentration. This facilitates interpretation of the results
and provides a better understanding of the significance of methemoglobin saturation
in specific cases. Based on an analysis of interferences, the presence of other
hemoglobin subforms or of endogenous components of plasma does not detract in
any way from the performance of the method.
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