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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 5, July/August 2002,
pp. 308-312
CASE REPORT: LC–MS Determination of Oxydemeton-methyl
and Its Main Metabolite Demeton-S-methylsulfon in Biological Specimens—Application
to a Forensic Case
J. Beike[1], Ch. Ortmann[1], Th. Meiners[2], B.
Brinkmann[1], and H. Köhler[1]
[1]Institute of Legal Medicine, University Hospital Münster, Röntgenstrasse,
D-48149 Münster, Germany and
[2]Institute of Pharmaceutical Chemistry, Westfälische Wilhelms-Universität,
Hittorfstrasse 58 - 62, D-48149 Münster, Germany
A sensitive method for the
identification and quantitation of the organophosphate insecticide oxydemeton-methyl
and its metabolite demeton-S-methylsulfon from human blood and various tissue
samples has been established. After solid-phase extraction using C18 cartridges
the extracts were analyzed by high-performance liquid chromatography with mass
selective detection (Finnigan MAT LCQ). The method was completely validated.
The limit of detection is 1 ng/g blood for the parent substance oxydemeton-methyl
and 2 ng/g blood for the metabolite demeton-S-methylsulfon. This method was
applied for the analysis of blood and tissue samples of a 58-year-old man who
died after the intake of Metasystox R-Spezial®, a product from Bayer containing
oxydemeton-methyl. Because of a prolonged interval between the ingestion and
death, the concentrations of oxydemeton-methyl and demeton-S-methylsulfon were
very low. Although the body was already putrefied when the autopsy was performed,
the chromatograms of blood and tissue samples were free of interfering peaks. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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