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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 5, July/August 2002,
pp. 280-285
Determination of Glycols in Biological Specimens by Gas
Chromatography–Mass Spectrometry
Vincent Gembus, Jean-Pierre Goullé, and
Christian Lacroix
Laboratoire de Pharmacocinétique et de Toxicologie Cliniques, Groupe
Hospitalier du Havre, B.P. 24 - 76083 Le Havre, France
A simple extraction and derivatization
procedure for the analysis of eight glycols (ethylene glycol, EG; diethylene
glycol, DEG; triethylene glycol, TEG; 1,2-propanediol, 1,2-PD; 1,3-propanediol,
1,3-PD; 1,2-butanediol, 1,2-BD; 2,3-butanediol, 2,3-BD; and hexylene glycol,
HXG) using a 2-µL serum or blood sample is described. Following deproteinisation
with acetonitrile, derivatization to its mono or di TMS derivative, glycols
were detected using gas chromatography-electron impact mass spectrometry equipped
with a split-spitless inlet and a DB-5MS column in the scan mode from 40 to
500 amu. Gamma-hydroxybutyrate-d6 (GHB-d6) was used as the internal standard.
The limits of detection and quantitation in 2 µL of serum ranged, respectively,
from 0.7 mg/L for EG to 8.5 mg/L for TEG and from 1.3 mg/L for EG to 18.2 mg/L
for 1,2-PD. A linear response was observed over the concentration range from
1 to 800 mg/L for EG and 18 from 800 for TEG and 1,2-PD for serum and blood.
Coefficients of variation for both intra-assay precision and interassay reproductibility
ranged respectively between 1.9% for TEG to 4.9% for 1,2-PD (11.8% for HXG)
and 3.5% for DEG to 9% for 2,3-BD (20.4 for HXG) at the 400 mg/L serum level.
The method was applied to plasma and whole blood. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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