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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 5, July/August 2002,
pp. 274-279
Application of EMIT® d.a.u.™ for the Semiquantitative
Screening of Methamphetamine Incorporated in Hair
Akihiro Miki*, Munehiro Katagi, and Hitoshi Tsuchihashi
Forensic Science Laboratory, Osaka Prefectural Police Headquarters, 1-3-18 Hommachi,
Chuo-ward, Osaka 541-0053, Japan
By the use of an enzyme multiplied
immunoassay technique (EMIT), a convenient and fairly sensitive semiquantitative
screening method was established for methamphetamine (MA) incorporated in hair.
MA in a 10-mg portion of hair was extracted into 5M HCl/methanol (1:20, v/v),
and the extract reconstituted in 100 µL water was assayed with double-concentrated
Emit d.a.u. Amphetamine Class assay reagents. The optimal cutoff concentration
of MA in hair was found by receiver operating characteristic analysis to be
1.0 ng/mg, and the detection limit was calculated to be 0.5 ng/mg. The semiquantitative
screening was possible over the concentration range from 1.0 to 200 ng/mg, and
the results are in good agreement with those by gas chromatographic–mass
spectrometric (GC–MS) determination. Although all positive results must
be confirmed by either GC–MS or a specific alternative methodology, this
method not only provided reliable screening suitable for drug enforcement purposes,
but it also allowed sectional profile analysis of MA in hair while requiring
only a 10-mg hair specimen. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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