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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 5, July/August 2002,
pp. 267-273
Multiplex Assay of Amphetamine, Methamphetamine, and Ecstasy
Drug using CEDIA® Technology
Rueyming Loor, Craig Lingenfelter, Prieya P. Wason,
Ken Tang, and David Davoudzadeh
Microgenics Corporation, 46360 Fremont Blvd., Fremont, California 94538
Using Microgenics unique technology,
cloned enzyme donor immunoassay (CEDIA), the multiplex assay (CEDIA Amphetamines/Ecstasy)
has been developed for the detection of amphetamine, methamphetamine, and ecstasy
drugs at cutoff level either of 500 ng/mL or 1000 ng/mL applicable for either
qualitative screening or semiquantitative measurement. The multiplex assay detects
the total concentration of amphetamine, methamphetamine, and ecstasy drugs in
urine samples. In addition, the assay detects metabolites of parent drugs and
structurally related drugs including d,l-amphetamine (67.2%), d,l-methamphetamine
(58.4%), MDA (113%), MDMA (199%), MDEA (207%), MBDB (123%), BDB (72%), PMA (24%),
and PMMA (100%). The assay is highly specific and exhibits minimal cross-reactivity
with the undesirable, structurally related over-the-counter amphetaminelike
drugs. Imprecision results (n = 120) demonstrate an intra-assay < 6.3% CV
and an interassay < 9.2% CV as performed by a modified NCCLS protocol. Using
the SAMHSA mandatory guidelines of a 500-ng/mL cutoff for the immunoassay and
a 250-ng/mL cutoff for GC–MS, the assay detects 164 out of 165 GC–MS-positive
samples. For drug abuse testing, the multiplex assay presents an excellent method
for the detection of multiple drugs with greater sensitivity and broader detection. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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