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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 5, July/August 2002,
pp. 245-252
Quantitation of Dialkyl Phosphate Metabolites of Organophosphate
Pesticides in Human Urine Using GC–MS–MS with Isotopic Internal Standards
Roberto Bravo, William J. Driskell, Ralph D. Whitehead
Jr., Larry L. Needham, and Dana B. Barr
Centers for Disease Control and Prevention, 4770 Buford Hwy. NE, Mailstop F-17,
Atlanta, Georgia 30341
Human exposure to organophosphate
pesticides can be estimated from the presence of urinary metabolites. An isotope-dilution
gas chromatography–tandem mass spectrometry (GC–MS–MS) method
was developed for quantitating the six dialkyl phosphate urinary metabolites
of at least 29 organophosphate pesticides. Urine samples were spiked with stable
isotope analogues of the dialkyl phosphates, evaporated using azeotropic distillation,
followed by chemical derivatization of the metabolites to their respective chloropropyl
phosphate esters. The chloropropyl phosphate esters were concentrated and then
analyzed using GC–MS–MS. The limits of detection (LODs) of the method
were in the low-to-mid picogram-per-milliliter range (parts per trillion) with
coefficients of variation of less than 20%. The use of stable isotope analogues
as internal standards for each of these metabolites allows for the highest degree
of accuracy and precision. Additionally, the low LODs allow the use of this
method in general population studies. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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