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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 3, April,
pp. 181-186
Technical Note Immunoassay Screening of Lysergic Acid Diethylamide
(LSD) and its Confirmation by HPLC and Fluorescence Detection Following LSD
ImmunElute Extraction
T. Grobosch and U. Lemm-Ahlers
Berliner Betrieb für Zentrale Gesundheitliche Aufgaben (BBGes), Klinische
Toxikologie und Pharmakologie, Berlin, Germany
In all, 3872 urine specimens
were screened for lysergic acid diethylamide (LSD) using the CEDIA DAU LSD assay.
Forty-eight samples, mainly from psychiatric patients or drug abusers, were
found to be LSD positive, but only 13 (27%) of these could be confirmed by high-performance
liquid chromatography with fluorescence detection (HPLC–FLD) following
immunoaffinity extraction (IAE). Additional analysis for LSD using the DPC Coat-a-Count
RIA was performed to compare the two immunoassay screening methods. Complete
agreement between the DPC RIA assay and HPLC–FLD results was observed at
concentrations below a cutoff concentration of 500 pg/mL. Samples that were
LSD positive in the CEDIA DAU assay but not confirmed by HPLC–FLD were
also investigated for interfering compounds using REMEDI HS drug-profiling system.
REMEDI HS analysis identified 15 compounds (parent drugs and metabolites) that
are believed to cross-react in the CEDIA DAU LSD assay: ambroxol, prilocaine,
pipamperone, diphenhydramine, metoclopramide, amitriptyline, doxepine, atracurium,
bupivacaine, doxylamine, lidocaine, mepivacaine, promethazine, ranitidine, and
tramadole. The IAE/HPLC–FLD combination is rapid, easy to perform and reliable.
It can reduce costs when standard, rather than more advanced, HPLC equipment
is used, especially for labs that perform analyses for LSD infrequently. The
chromatographic analysis of LSD, nor-LSD, and iso-LSD is not influenced by any
of the tested cross-reacting compounds even at a concentration of 100 ng/mL. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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