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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 3, April,
pp. 176-180
Technical Note: Method for the Determination of Dialkyl
Phosphates in Urine by Strong Anion Exchange Disk Extraction and In-Vial Derivatization
W.C. Lin[1], C.H. Kuei[2], H.C. Wu[2], C.C. Yang[3],
and H.Y. Chang[3]
[1]Department of Cosmetic Science, Chia Nan University of Pharmacy and Science,
Tainan, Taiwan;
[2]Department of Chemistry, National Cheng Kung University, Tainan, Taiwan;
and
[3]Graduate Institute of Environmental and Occupational Health, College of Medicine,
National Cheng Kung University, Tainan, Taiwan
A method for the determination
of four dialkylphosphate metabolites in urine by strong anion exchange disk
(SAX) was investigated. Calcium hydroxide was added to a 1-mL urine sample to
reduce interference. The aliquot was passed through the SAX disk to accumulate
dialkylphosphate metabolites on the disk. The retained dialkylphosphate metabolites
were derivatized with methyl iodide in acetonitrile online, and the resulting
methyl esters of dialkylphosphate metabolites were directly analyzed by capillary
column gas chromatography with flame photometric detection. The recoveries of
these dialkylphosphate metabolites were found to be stable. When the intact
sample was diluted with deionized water at a 1:1 ratio, the recoveries were
both increased and stabilized. The urine samples collected from eight fruit
farmers showed that levels of dialkylphosphate metabolites in urine were significantly
different before and after pesticide application, indicating the method established
in this study is applicable for real sample analysis. Compared with previous
studies, this method not only can greatly simplify sample preparation, but it
can also significantly reduce the consumption of toxic solvents in sample preparation.. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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