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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 26, Number 3, April,
pp. 138-143
Gas Chromatography–Mass Spectrometry Analysis of 4-O-Methylpyridoxine
(MPN) in the Serum of Patients with Ginkgo Seed Poisoning
Manami Fujisawa[1],[2], Yasushi Hori[1],[2], Mariko
Nakajima[1],[2], Kenji Shimada[1], Hideto Yoshikawa[3], and Keiji Wada[4]
[1]Department of Analytical Chemistry, Niigata College of Pharmacy, Niigata,
Japan;
[2]Section of Analytical Toxicology, Niigata City General Hospital, Niigata,
Japan;
[3]Department of Pediatrics, Niigata City General Hospital, Niigata, Japan;
and
[4]Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Health
Sciences University of Hokkaido, Hokkaido, Japan
The 4-O-methylpyridoxine
(MPN) present in the seeds of the Ginkgo biloba (maidenhair tree) has anti-vitamin
B6 actions, and ginkgo seed poisoning can induce convulsions. We developed a
specific quantitative method using gas chromatography–mass spectrometry
for the analysis of MPN in human serum. The trifluoroacyl (TFA) derivative of
MPN was obtained by treating MPN with trifluoroacetic anhydride at 50°C
for 5 min and remained stable for 6 h. The calibration curve of standard MPN
obtained in the selective ion mode using the base ion (m/z 343) was linear between
100 pg and 10 ng, and the detection limit was 50 pg. The full mass spectrum
of 100 pg of the TFA derivative of MPN was obtained easily. MPN was extracted
from the serum with the use of a C18 solid-phase extraction cartridge. The recovery
rate of MPN added to the serum at a concentration of 0.1 µg/mL was 90.0%. Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
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