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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 25, Number 7, October, pp.555-558
The Determination of 11-nor-D9-Tetrahydrocannabinol-9-Carboxylic
Acid (THC-COOH) in Hair using Negative Ion Gas Chromatography–Mass Spectrometry
and High-Volume Injection
Christine Moore and Frank Guzaldo
U.S. Drug Testing Laboratories, 1700 S. Mount Prospect Road, Des Plaines, Illinois
60018
Thomas Donahue
Omega Laboratories, 400 N. Cleveland Avenue, Mogadore, Ohio 44260
The determination of 11-nor-D9-THC-9-carboxylic acid (THC-COOH)
in hair specimens at the sensitivity required to detect marijuana users is a
difficult analytical problem. A sensitive and specific method has been developed
for the quantitative assay of THC-COOH in hair. Hair specimens were washed,
incubated in sodium hydroxide, subjected to solid-phase extraction, and analyzed
using high-volume injection coupled with negative chemical ionization (NCI)
mass spectrometry. A common disadvantage of chemical ionization, the production
of a single mass-to-charge ratio ion, was also addressed. By specific selection
of the derivatizing agent, three ions were monitored, allowing the calculation
of two ion ratios, as in electron impact mode. The method was applied to several
hair specimens taken from known marijuana users and workplace specimens. This
is the first publication describing the use of high-volume injection and NCI
mass spectrometry for the determination of THC-COOH in hair.
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