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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 25, Number 7, October, pp. 520-524
Gas Chromatography–Mass Spectrometry Confirmation
of Cozart RapiScan Saliva Methadone and Opiates Tests
Lesley Moore[1], John Wicks[2], Vina Spiehler[3],
and Richard Holgate[4]
[1]Cozart Bioscience Ltd., Abingdon, Oxfordshire, United Kingdom; [2]Tricho-Tech,
Cardiff, Wales, United Kingdom; [3]Spiehler & Associates, Newport Beach,
California; and [4]Ferry Cross Substance Misuse Services, Norwich, United Kingdom
The object of this study was to determine the sensitivity and
specificity of the Cozart RapiScan onsite saliva test for methadone and opiates
versus laboratory-based enzyme immunoassay (EIA) and gas chromatography–mass
spectrometry (GC–MS) confirmation. Fifty saliva specimens were obtained
from 28 volunteers among persons entering a substance abuse clinic. Specimens
were tested onsite using the Cozart RapiScan Saliva test and Cozart RapiScan
Reader. Specimens were retested by Cozart Microplate EIA assays on receipt at
the laboratory and then frozen for later confirmation by GC–MS. For GC–MS,
deuterated internal standards were added to specimen aliquots which were extracted
using solid-phase columns at pH 6 and eluted with dichloromethane/isopropanol/ammonia
(80:19:2). The dry residues were derivatized with PFOH and PFPA and dried, and
the reconstituted extract was injected and quantitated by GC–MS. The Cozart
RapiScan Methadone Saliva Assay was found to have a sensitivity and specificity
of 100% ± 12% versus GC–MS (2-ng/mL cutoff) and a sensitivity of
100% ± 11% and a specificity of 95% ± 2.4% versus the Microplate
EIA for methadone (30-ng/mL cutoff). The Cozart RapiScan Saliva Opiate test
had a sensitivity of 100% ± 12% and a specificity of 92% ± 3.2%
versus GC–MS (2-ng/mL cutoff) and a sensitivity of 96% ± 2.2% and
specificity of 95% ± 2.4% versus the Microplate EIA for opiates (30-ng/mL
cutoff).
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